Especially in dermatology, amniotic membrane transplantation (AMT) has become firmly established as a skin substitute for the treatment of burns and chronic wounds (e.g. diabetic foot). The membrane consists of an epithelial layer, basement membrane and stroma and can be applied in two orientations.
The epithelial side faces the embryo. Its surface is smoother than that of the rougher stromal side, which faces the outer amniotic membrane. When the graft is delivered, the stromal side is positioned towards the support material as standard, while the epithelial side is open.
The graft is delivered cryopreserved and should be allowed to thaw at room temperature for at least 30 minutes before use.